Coding
Part:BBa_K2637047:Design
Designed by: Jiangnan Xie Group: iGEM18_Tianjin (2018-10-05)
NanoLuc with TDH3 Promoter and ADH1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 366
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added the constitutive TDH3 promoter and ADH1 terminator to the Part:BBa_K1680009 to make the cassette which can express NanoLuc continuously.
Source
The gene NanoLuc derives from Part:BBa_K1680009. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.