Coding

Part:BBa_K2637047:Design

Designed by: Jiangnan Xie   Group: iGEM18_Tianjin   (2018-10-05)


NanoLuc with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added the constitutive TDH3 promoter and ADH1 terminator to the Part:BBa_K1680009 to make the cassette which can express NanoLuc continuously.


Source

The gene NanoLuc derives from Part:BBa_K1680009. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.

References